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STANDARD

TEST ORGANISMS

STANDARD CONDITIONS

EN 13624:

 

Suspension test

C. albicans

A. brasiliensis

Temperature

Hygienic Handrub

20OC

Surgical Handrub

20OC

Surface product

4OC- 30OC

Instrument disinfectant

20OC- 70OC

Contact time

Hygienic Handrub

30 secs- 60 secs

Surgical Handrub

 1 min- 5 mins

Surface product

  5 minutes

Instrument disinfectant

 ≤ 60 minutes

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin + 3.0 ml sheep erythrocytes

Concentrations

80% 50% 10%

EN 13727:

 

Suspension test

P. aeruginosa

S. aureus

E. hirae

E. coli K12 (Hand products only)

Temperature

Hygienic Handrub

20OC

Surgical Handrub

20OC

Surface product

4OC- 30OC

Instrument disinfectant

20OC- 70OC

Contact time

Hygienic Handrub

30 secs- 60 secs

Surgical Handrub

1 min- 5 mins

Surface product

5 minutes

Instrument disinfectant

60 minutes

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin + 3.0 ml sheep erythrocytes

Concentrations

80% (or 97%) 50% 10%

*10% cost increase on tests performed at 97%

EN 14348:

 

Suspension test

M. avium

M. terrae

Temperature

20OC

Contact time

Instrument disinfectant

<60 minutes

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin + 3.0 ml sheep erythrocytes

Concentrations

80% 50% 10%

EN 14476:

VIROLOGY

 

Suspension test

Poliovirus-1

Adenovirus-5

Murine norovirus Vaccinia Virus

Temperature

Hygienic Handrub/hand wash

20OC

Surface product

4OC- 30OC

Instrument disinfectant

20OC- 70OC

Contact time

Hygienic Handrub/hand wash

30 – 120 seconds

Surface product

<5 or <60 minutes

Instrument disinfectant

<60 minutes

Clean conditions

0.3 g/l bovine albumin- minimum for hand rub

Dirty conditions

3.0 g/l bovine albumin + 3.0 ml sheep erythrocytes- minimum for hand wash

Concentrations

80% (or 97%*) 50% 10%

*20% cost increase on tests performed at 97%. Does not include Murine norovirus and Vaccinia virus

EN 14561:

 

Glass surface test

P. aeruginosa

S. aureus

E. hirae

Temperature

20OC

Contact Time

Instrument disinfectant

<60 minutes

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin + 3.0 ml sheep erythrocytes

Concentrations

100% 50% 10%

 



EN 14562:

 

Glass surface test

C. albicans

A. brasiliensis

Temperature

20OC

Contact Time

Instrument disinfectant

<60 minutes

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin + 3.0 ml sheep erythrocytes

Concentrations

100% 50% 10%

EN 14563:

 

Glass surface test

M. avium

M. terrae

Temperature

20OC

Contact Time

Instrument disinfectant

<60 minutes

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin + 3.0 ml sheep erythrocytes

Concentrations

100% 50% 10%

EN 16615:

 

Surface test

P. aeruginosa

S. aureus

E. hirae

C. albicans

 

Temperature

4OC - 30OC

Contact Time

No less than 1 minute, no greater than 60 minutes

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin + 3.0 ml sheep erythrocytes


 

STANDARD

TEST ORGANISMS

STANDARD CONDITIONS

EN 1040:

 

Suspension test

P. aeruginosa

S. aureus

Temperature

20OC

Contact time

5 minutes

Clean conditions

None

Dirty conditions

None

Concentrations

80% 50% 10%

EN 1275:

 

Suspension test

C. albicans

A. brasiliensis

Temperature

20OC

Contact time

15 minutes

Clean conditions

None

Dirty conditions

None

Concentrations

80% 50% 10%

EN 1276:

 

Suspension test – 

P. aeruginosa

S. aureus

E. hirae

E. coli

E. faecium (temp 40°C)

Temperature

4°C - 60°C (typically 20°C)

Contact time

1 min - 60 mins (typically 5 mins)

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin

Concentrations

80% 50% 10%

EN 1276:

 

Suspension test – hand hygiene products only

P. aeruginosa

S. aureus

E. hirae

E. coli K12

Temperature

20°C

Contact time

30 s or 60 s

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin

Concentrations

80% 50% 10% (handwash products diluted 50% v/v first)

EN 1650:

 

Suspension test

C. albicans

A. brasiliensis

Temperature

4°C - 40°C (typically 20°C)

Contact time

1 min - 60 mins (typically 15 mins)

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin

Concentrations

80% 50% 10%

EN 1650:

 

Suspension test – hand hygiene products only

C. albicans

 

Temperature

20°C

Contact time

30 s or 60 s

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin

Concentrations

80% 50% 10% (handwash products diluted 50% v/v first)

EN 13704:

 

Suspension test

B. subtilis

Temperature

20OC

Contact time

60 minutes

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

N/A

Concentrations

80% 50% 10%

EN 13697:

(Bacteria)

Surface test

P. aeruginosa

S. aureus

E. hirae

E. coli

Temperature

4°C - 40°C (typically 20°C)

Contact time

1 min - 60 mins (typically 5 mins)

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin

Concentrations

100% 50% 10%

EN 13697:

(Fungi)

Surface test

C. albicans

A. brasiliensis

Temperature

4°C - 40°C (typically 20°C)

Contact time

1 min - 60 mins (typically 15 mins)

Clean conditions

0.3 g/l bovine albumin

Dirty conditions

3.0 g/l bovine albumin

Concentrations

100% 50% 10%

 

 

 

GENERAL PURPOSE AREA (continued): Bacteria, Fungi, bacterial endospores, Hand rubs / washes

NOTE: Hand wash products are diluted to 50.0% as standard

 

EN 13610: 

(Bacteriophage)

Suspension test

L. lactis phage P001

L. lactis phage P008

Temperature

4°C - 40°C (typically 20°C)

 

Contact time

15 minutes

 

Interfering substance (standard)

10% acidic whey solution

 

Interfering substance (optional)

10% skim milk

 

Concentrations

As requested

 

EN 1499:

 

Hand trial – Hand wash products

E. coli K12

Temperature

20OC

Contact time

No less than 30 seconds: 

No more than 60 seconds

EN 1500:

 

Hand trial – Hand rub products

E. coli K12

Temperature

20OC

Contact time

No less than 30 seconds: 

No more than 60 seconds

 

VETERINARY AREA: Bacteria, Fungi, Mycobacteria, Virus

STANDARD

TEST ORGANISMS

STANDARD CONDITIONS

EN 1656:

 

Suspension test

P. aeruginosa

S. aureus

E. hirae

P. vulgaris

Temperature

10OC

Contact time

30 minutes

Clean conditions

3.0 g/l bovine albumin

Dirty conditions

10.0 g/l bovine albumin + 10.0 g/l yeast extract

Concentrations

80% 50% 10%

EN 1656:

Teat Disinfectant Only

Suspension test

E. coli

S. aureus

S. uberis

Temperature

30OC

Contact time

5 minutes

Soiling conditions

10.0 g/l skimmed milk

Concentrations

80% 50% 10%

EN 1657:

 

Suspension test

C. albicans

A. brasiliensis

Temperature

10OC

Contact time

30 minutes

Clean conditions

3.0 g/l bovine albumin

Dirty conditions

10.0 g/l bovine albumin + 10.0 g/l yeast extract

Concentrations

80% 50% 10%

EN 1657:

Teat Disinfectant Only

Suspension test

C. albicans

 

Temperature

30OC

Contact time

5 minutes for post milking

10 minutes for premilking

Soiling conditions

10.0 g/l skimmed milk

Concentrations

80% 50% 10%

EN 14204:

 

Suspension test

M. avium

Temperature

10OC

Contact time

60 minutes

Clean conditions

3.0 g/l bovine albumin

Dirty conditions

10.0 g/l bovine albumin + 10.0 g/l yeast extract

Concentrations

80% 50% 10%

EN 14675:

VIROLOGY

 

Suspension test

Bovine enterovirus-1

(ECBO)

Temperature

10OC

Contact time

30 minutes

Clean conditions

3.0 g/l bovine albumin

Dirty conditions

10.0 g/l bovine albumin + 10.0 g/l yeast extract

Concentrations

80% 50% 10%

EN 14349:

 

Surface test

P. aeruginosa

S. aureus

E. hirae

P. vulgaris

Temperature

10OC

Contact time

30 minutes

Clean conditions

3.0 g/l bovine albumin

Dirty conditions

10.0 g/l bovine albumin + 10.0 g/l yeast extract

Concentrations

100% 50% 10%

EN 16437:

 

Surface test

P. aeruginosa

S. aureus

E. hirae

P. vulgaris

Temperature

10OC

Contact time

60 minutes

Soiling Conditions

3.0 g/l bovine albumin

Concentrations

100% 50% 10%

EN 16438:

 

Surface test

C. albicans

A. brasiliensis

Temperature

10OC

Contact time

60 minutes

Clean conditions

3.0 g/l bovine albumin

Dirty conditions

10.0 g/l bovine albumin + 10.0 g/l yeast extract

Concentrations

100% 50% 10%